2/16/2023 0 Comments Manuscript pens![]() ![]() ![]() Determination of carbohydrates by anion exchange chromatography with pulsed amperometric detection. Starch digested product analysis by HPAEC reveals structural specificity of flavonoids in the inhibition of mammalian alpha-amylase and alpha-glucosidases. Indirect chronic effects of an oleuropein-rich olive leaf extract on sucrase-isomaltase in vitro and in vivo. Antidiabetic activity of Sedum dendroideum: metabolic enzymes as putative targets for the bioactive flavonoid kaempferitrin. Citrus polyphenols and risk of type 2 diabetes: evidence from mechanistic studies. Dietary phenolic compounds selectively inhibit the individual subunits of maltase-glucoamylase and sucrase-isomaltase with the potential of modulating glucose release. Simsek, M., Quezada-Calvillo, R., Ferruzzi, M. ![]() Characterization of mucosal disaccharidases from human intestine. Contribution of mucosal maltase-glucoamylase activities to mouse small intestinal starch alpha-glucogenesis. Inhibition of human and rat sucrase and maltase activities to assess antiglycemic potential: optimization of the assay using acarbose and polyphenols. Different sucrase-isomaltase response of Caco-2 cells to glucose and maltose suggests dietary maltose sensing. Amino acids interference on the quantification of reducing sugars by the 3,5-dinitrosalicylic acid assay mislead carbohydrase activity measurements. Chain-length dependence of alkaline 3,5-dinitrosalicylate and chain-length independence of alkaline copper. Reducing value methods for maltodextrins. Iodine-maltosaccharide complexes: relation between chain-length and colour. Multi-branched nature of amylose and the action of debranching enzymes. Inhibition of human α-amylase by dietary polyphenols. Critical review on conventional spectroscopic alpha-amylase activity detection methods: merits, demerits, and future prospects. Maltoheptaoside hydrolysis with chromatographic detection and starch hydrolysis with reducing sugar analysis: comparison of assays allows assessment of the roles of direct alpha-amylase inhibition and starch complexation. Naringenin inhibits alpha-glucosidase activity: a promising strategy for the regulation of postprandial hyperglycemia in high fat diet fed streptozotocin induced diabetic rats. Flavonoids in the treatment of diabetes clinical outcomes and mechanism to ameliorate blood glucose levels. Polyphenols and their effects on diabetes management: a review. in StatPearls (StatPearls Publishing, 2022) Īryaeian, N., Sedehi, S. Dietary glycemic index and load and the risk of type 2 diabetes: assessment of causal relations. Process for producing a high-purity isomaltose. Direct starch digestion by sucrase-isomaltase and maltase-glucoamylase. Structural studies of the intestinal alpha-glucosidases, maltase-glucoamylase and sucrase-isomaltase. Flavonoids as human intestinal α-glucosidase inhibitors. A full dataset therefore takes 1–3 d and allows detection of subtle changes in enzyme activity with high sensitivity and reliability. The wet-lab assay takes ~2–5 h depending on the number of samples, and the HPAE-PAD analysis takes 35 min per sample. Multiple enzyme sources can be used, but here we present the protocol using commercially available human α-amylase to assess starch hydrolysis with maltoheptaose as the substrate, and with brush border sucrase-isomaltase (with maltase, sucrase and isomaltase activities) derived from differentiated human intestinal Caco-2(/TC7) cells to assess hydrolysis of disaccharides. The assay is especially suitable for measuring inhibition by compounds, drugs and extracts, with minimal interference from impurities or endogenous components, because the substrates and digestive products in the enzyme activity assays are quantified directly by high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD). Here, we present an in vitro protocol developed to accurately and specifically assess the activity of α-amylases and α-glucosidases, including sucrase, maltase and isomaltase. Furthermore, delaying carbohydrate digestion via inhibition of α-amylases and α-glucosidases is an effective strategy to blunt blood glucose spikes, a major risk factor for developing metabolic diseases. Inhibition of these enzymes is the major activity of the drugs acarbose and miglitol, which are used to manage diabetes. Carbohydrate digestion in the mammalian gastrointestinal tract is catalyzed by α-amylases and α-glucosidases to produce monosaccharides for absorption.
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